Summary
Type: SPE
Application #: 300810
Catalog#: 730011 - SPE Cartridges, Chromabond, C18 ec, 1ml, PP, 100mg, 100/pk
Detection:
Conditions: Column conditioning:
5 ml acetonitrile, then 5 ml dist. water
Sample application:
Slowly force or aspirate the sample through the column
Column washing:
20 ml water, then 4 – 5 ml acetonitrile / water (1 : 1, v/v); thoroughly dry
the column under vacuum
Elution:
2 – 3 x 500 μl acetonitrile / 0.05 M phosphate buffer pH 5.8 (3 : 2, v/v)
Authors: Macherey-Nagel
Source: LC department